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Coding

Part:BBa_K1085005:Design

Designed by: Claudio Tiecher   Group: iGEM13_Groningen   (2013-09-10)

RBS Start-codon SpiderSilkSubunitN1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 26
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The DNA sequence was codon-optimized for Bacillus subtilis. Moreover the nucleotide sequence was optimized in order to accomplish the production standard of IDT. A couple of optimized codons were affected by this.


Source

The amino acids sequence of the silk protein was obtained from MaSp2 of the Argiope aurantia. The DNA sequence was synthesized by Integrated DNA Technologies (IDT).


References